Multiplex PCR-Based Method for Identification of Common Clinical Serotypes of Salmonella enterica subsp. enterica
نویسندگان
چکیده
منابع مشابه
A Multiplex PCR based Method for the Identification of common Clinical Serotypes of Salmonella enterica subspecies enterica
A multiplex PCR method has been developed to differentiate between the most common clinical serotypes of Salmonella enterica subsp. enterica encountered in Washington State and the USA in general. Six genetic loci from S. Typhimurium and four from S. Typhi were used to create an assay consisting of two five-plex PCR reactions. The assays gave reproducible results with 30 different serotypes tha...
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BACKGROUND & OBJECTIVES A rapid and simple alternative method is needed to replace the laborious, time-consuming Salmonella serotyping. The objective of the present study was to improve and simplify a previously reported multiplex polymerase chain reaction (PCR)-based method and to create an online server to enable rapid determination of serovars. METHODS A method of multiplex PCR-based genom...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2006
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00701-06